ISSN 1556-6757


SJI 


 

 

 

 

 
 

Volume 5, Issue 1, 2012

 

Development of the Quantitative Real-Time PCR Assay for the Detection of Mycobacterium xenopi.  Rahizan Issa, Athirah Abdul Sani, Nurul Akma Mohd Hassan, and Norazah Ahmad

Abstract
A quantitative real-time polymerase chain reaction (qPCR) assay using SYBR Green I dye was developed for the detection of the internal transcribed spacer (ITS) of 16S-23S rDNA region in Mycobacterium xenopi (M. xenopi). The qPCR assay for the detection of the M. xenopi DNA had a Cp at cycle 14.17 0.07 and a Tm of 90.23 0.03C. As for other mycobacterial species, the Cp and Tm were above cycle 21 and below 86C, respectively. The Tm value of 89.47 to 90.03C could be used to detect M. xenopi and differentiate M. xenopi from other mycobacterial species. Full Article



 

Prime risk factor of cardiovascular diseases and weight loss program in the Kazakh population: A panel study 


 

Kuat Oshakbayev, Zhangentkhan Abylaiuly, Baglan Zhumabekova, Meruyert Gazaliyeva, Bibazhar Dukenbayeva, Pernekul Oshakbayev

Abstract
This panel study discovered the rank of risk factors (RFs) for cardiovascular diseases (CVD) such as arterial hypertension (AH) and coronary artery disease (CAD), and studied the results of a weight loss program. The research included 1662 people. Results showed that the prevalence of CVD and non-cardiological therapeutic diseases increased almost 2-3-fold in the group with overweight. The basic and prime RF was overweight and weight loss led to reduction of CVD symptoms as well as a decrease of tobacco, alcohol and salt consumption, and the improvement of individual physical capability. Weight loss was only due to fatty mass reduction. Full Article